Bacteriological and Molecular Study Characterization of Klebsiella pneumoniae Isolated from Different Clinical Specimens
DOI:
https://doi.org/10.25079/ukhjse.v3n2y2019.pp22-30Keywords:
Klebsiella pneumoniae, ESBL, antibiotic susceptibility, UTIAbstract
Klebsiella pneumoniae is one of the foremost imperative opportunistic pathogens. Urinary characteristic disease is the common infectious bacterial contamination caused by K. pneumonia, that are rising around the world comprising a danger to community and clinic settings. K. pneumonia Isolates were evaluated for their antimicrobial susceptibility. Samples were taken from 80 patients with diverse diseases infection. Genomic DNA of K. pneumonia Confines were extricated and detection of ESBL Genes was 53.75% of the isolates were predominance for ESBL Genes blaTEM, blaSHV and bla CTX-M 82.5, 92.5 and 70 %, respectively. Out of 100 obtained clinical isolates of K. pneumonia from diverse healing centers and therapeutic research facilities in Duhok/ Iraq as it were (80%) isolates had a place to the genus K. pneumonia. Ampicillin and Aztreonam 100 % anti-microbial resistance where was Imipenem Ertapenem Meropenem 100% sensitive. Conveyance of ESBLs creating K. pneumoniae among different clinical tests because it was 71.42% in urine, 40. 90 % in wound swabs, 42. 10 % in sputum and 50 % in blood culture. The recurrence of the ESBL production can easily be thought little of within the clinical isolates of K. pneumoniae with the utilize of the current CLSI suggested strategies, an ideal recognizable proof of the ESBL creating isolates is basic to define approaches for an experimental antimicrobial treatment
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Amr, M., Hady El Gilany, A. & El-Hawary, A. (2008). Does gender predict medical students’ stress in Mansoura, Egypt. Medical Education Online, 13, 12.
Bradford, P. A. (2001). Extended-spectrum beta-lactamases in the 21st century: Characterization, epidemiology, and detection of this important resistance threat. Clinical Microbiology Reviews, 14(4), 933-951.
Bradford, P. A., Cherubin, C. E., Idemyor, V., Rasmussen, B. A. &Bush, K. (1994). Multiply resistant Klebsiella pneumoniae strains from two Chicago hospitals: Identification of the extended-spectrum TEM-12 and TEM-10 ceftazidime-hydrolyzing beta-lactamases in a single isolate. Antimicrobial Agents and Chemotherapy, 38(4), 761-766.
Bush, K. (2002).The impact of beta-lactamases on the development of novel antimicrobial agents. Current Opinion in Investigational Drugs, 3(9), 1284-1290.
Chaudhary, U. & Aggarwal, R. (2004). Extended spectrum -lactamases (ESBL) - an emerging threat to clinical therapeutics. Indian Journal of Medical Microbiology, 22(2), 75-80.
Cheesbrough, M. (2006). District Laboratory Practice in Tropical Countries. Part 2, 2nd Edition. Cambridge University.
Falagas, M. E. & Karageorgopoulos, D. E. (2009). Extended-spectrum beta-lactamase-producing organisms. Journal of Hospital Infection, 73(4), 345-354.
Feizabadi, M .M., Etemadi, G., Yadegarinia, D., Rahmati, M., Shabanpoor, S. & Bokaei, S. (2006). Antibiotic-resistance patterns and frequency of extended-spectrum beta-lactamase-producing isolates of Klebsiella pneumoniae in Tehran. Medical Science Monitor, 12(11), BR362-BR365.
Golamreza, I. & Moghadas, S. A. J. (2010). Frequency of extended-spectrum beta lactamase positive and multidrug resistance pattern in ram-negative urinary isolates, Semnan, Iran. Jundishapur Journal of Microbiology, 3(3), 107-113.
Huseyin, T. & Hakki, B. I. (2005). Molecular characterization of TEM- and SHV-derived extended-spectrum beta-lactamases in hospital-based Enterobacteriaceae in Turkey. Japanese Journal of Infectious Diseases, 58(3), 162-167.
Kahan, N. R., Chinitz, D. P., Waitman, D. A., Dushnitzky, D., Kahan, E. & Shapiro, M. (2006). Empiric treatment of uncomplicated urinary tract infection with fluoroquinolones in older women in Israel: Another lost treatment option? Annals of Pharmacotherapy, 40(12), 2223-2227.
Kiesser, T. (1995). Preparation and Analysis of Genomic and Plasmid DNA. Norwich: John Innes Center. 17, p. 123-129.
Knothe, H., Shah, P., Krcmery, V., Antal, M. & Mitsuhashi, S. (1983). Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. Infection, 11(6), 315-317.
MacFaddin, J. F. (1999). Biochemical Tests for Identification of Medical Bacteria. 3rd Edition. Philadelphia: Lippincott Williams & Wilkins.
Manikandan, C. & Amsath, A. (2013). Antibiotic susceptibility pattern of Klebsiella pneumoniae isolated from urine samples. International Journal of Current Microbiology and Applied Sciences., 2(8), 330-337.
Mansouri, M. & Ramazanzadeh, R. (2009). Spread of extended-spectrum beta-lactamase producing Escherichia coli clinical isolates in Sanandaj Hospitals. Journal of Biological Sciences, 9(4), 362-366.
Paterson, D. L. & Bonomo, R. A. (2005). Extended-spectrum beta lactamases: A clinical update. Clinical Microbiology Reviews, 18(4), 657–686.
Paterson, D. L. & Bonomo, R. A. (2005). Extended-spectrum beta-lactamases: A clinical update. Clinical Microbiology Reviews, 18(4), 657-686.
Sambrook, J. & Russell, D. W. (2001.). Molecular Cloning. 3rd Edition. New York: Cold Spring Harbor Laboratory Press.
Shahcheraghi, F., Moezi, H. & Feizabadi, M. M. (2007). Distribution of TEM and SHV beta-lactamase genes among Klebsiella pneumoniae strains isolated from patients in Tehran. Medical Science Monitor, 13(11), BR247-BR250.
Shannon, K. & Phillips, I. (1986). The effects on beta-lactam susceptibility of phenotypic induction and genotypic derepression of beta-lactamase synthesis. Journal of Antimicrobial Chemotherapy, 18, 15-22.
Sobhan, G., Zamberi, S., Nourkhoda, S., Reza, M., Vasantah, K. N., Abbas, M., Ali, H., Mohammad, R., Mohammad, R. & Reza, R. (2011). The prevalence of ESBLs producing Klebsiella pneumoniae isolates in some major hospitals, Iran. The Open Microbiology Journal, 5, 91-95.
Winokur, P. L., Brueggemann, A., DeSalvo, D. L., Hoffmann, L., Apley, M. D., Uhlenhopp, E. K., Pfaller, M. A. & Doern, G. V. (2000). Animal and human multidrug-resistant, cephalosporin-resistant salmonella isolates expressing a plasmid-mediated CMY-2 AmpC beta-lactamase. Antimicrobial Agents and Chemotherapy, 44(10), 2777-2783.
Yu, Y., Zhou, W., Chen, Y., Ding, Y. & Ma, Y. (2002). Epidemiological and antibiotic resistant study on extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Zhejiang Province. Chinese Medical Journal, 115(10), 1479-1482.
Zhang, W., Luo, Y., Li, J., Lin, L., Ma, Y. Hu, C., Jin, S., Ran, L. & Cui, S. (2011). Wide dissemination of multidrug-resistant Shigella isolates in China. Journal of Antimicrobial Chemotherapy, 66(11), 2527-2535.
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