Bacteriological and Molecular Study Characterization of Klebsiella pneumoniae Isolated from Different Clinical Specimens

Authors

  • Marwan Khalil Qader Department of Biology, College of Science, University of Duhok, Duhok, Kurdistan Region - F.R. Iraq

DOI:

https://doi.org/10.25079/ukhjse.v3n2y2019.pp22-30

Keywords:

Klebsiella pneumoniae, ESBL, antibiotic susceptibility, UTI

Abstract

Klebsiella pneumoniae is one of the foremost imperative opportunistic pathogens. Urinary characteristic disease is the common infectious bacterial contamination caused  by K. pneumonia, that  are rising around the world comprising a danger to community and clinic settings. K. pneumonia Isolates were  evaluated for their antimicrobial susceptibility. Samples were taken from 80 patients with  diverse diseases  infection. Genomic DNA of K. pneumonia Confines were extricated  and detection of ESBL Genes was 53.75% of the isolates  were predominance for ESBL  Genes blaTEM, blaSHV and bla CTX-M 82.5, 92.5  and  70 %, respectively. Out of 100 obtained clinical isolates of K. pneumonia from diverse healing centers and therapeutic research facilities in Duhok/ Iraq as it were (80%) isolates had a place to the genus K. pneumonia. Ampicillin and Aztreonam 100 % anti-microbial resistance where was Imipenem Ertapenem Meropenem 100% sensitive. Conveyance of ESBLs creating K. pneumoniae among different clinical tests because it was 71.42% in urine, 40. 90 % in wound swabs, 42. 10 % in sputum and 50 % in blood culture. The recurrence of the ESBL production can easily be thought little of within the clinical isolates of K. pneumoniae with the utilize of the current CLSI suggested strategies, an ideal recognizable proof of the ESBL creating isolates is basic to define approaches for an experimental antimicrobial treatment  

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Author Biography

  • Marwan Khalil Qader, Department of Biology, College of Science, University of Duhok, Duhok, Kurdistan Region - F.R. Iraq

    Mr. Marwan Khalil Qader is currently a lecturer at the Department of Biology, College of Science, University of Duhok, Kurdistan Region, F.R. Iraq. He obtained an M.Sc. in Biology, Molecular Microbiology, from College of Education, University of Duhok in 2010. He graduated from the College of Science, University of Mosul, F.R. Iraq in 2005 with a B.Sc. in Biology. His areas of interest are: Molecular biology and Microbiology.

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Published

2019-12-27

Issue

Section

Research Articles

How to Cite

Bacteriological and Molecular Study Characterization of Klebsiella pneumoniae Isolated from Different Clinical Specimens. (2019). UKH Journal of Science and Engineering, 3(2), 22-30. https://doi.org/10.25079/ukhjse.v3n2y2019.pp22-30